![]() ![]() The monitoring of XBP1 mRNA splicing can be performed using RT-PCR, as illustrated in Fig. IRE1α endoribonuclease activity is involved in two major mechanisms, which are the unconventional splicing of XBP1 mRNA and the degradation of mRNA substrates ( Hollien and Weissman, 2006 Hollien et al., 2009 Ron and Walter, 2007). Indeed, upon accumulation of misfolded proteins in the ER, IRE1α oligomerizes and trans-autophosphorylates, resulting in the activation of its endoribonuclease activity. XBP1 mRNA splicing is an event downstream of IRE1α activation. Eric Chevet, in Methods in Enzymology, 2011 3.1.2 Splicing of XBP1 mRNA
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